Cell culture supernates, serum, bone tissue, tissue.

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rabbit BMP-5.

<10pg/ml

156pg/ml-10,000g/ml

Recognizes natural and recombinant rabbit BMP-5. There is no detectable cross-reactivity with other relevant proteins.

This Rabbit BMP-5 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit BMP-5 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit BMP-5 amount of sample captured in plate.

96-well plate precoated with anti-Rabbit BMP-5 antibody, 1x plate Lyophilized recombinant Rabbit BMP-5 standard, 2x10ng/tube Biotinylated anti-Rabbit BMP-5 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.