The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-alpha, and down-regulated by IFN-gamma.
Sample Type
Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA).
Intended Use
Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Mouse FCRN/FCGRT.
Range
93.7pg/ml-6000pg/ml
Specificity
Recognizes natural and recombinant Mouse FCRN/FCGRT. There is no detectable cross-reactivity with other relevant proteins.
Test Principle
This Mouse FCRN/FCGRT BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for FCRN/FCGRT has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: S22-S297) and test samples are added to the wells, a biotinylated detection specific for FCRN/FCGRT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse FCRN/FCGRT amount of sample captured in plate.
Kit Components
96-well plate precoated with anti-Mouse Fcgrt antibody, 1x plate Lyophilized recombinant Mouse Fcgrt standard, 2x10ng/tube Biotinylated anti-Mouse Fcgrt antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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