Cell culture supernates, serum, plasma (Heparin or EDTA).

Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Equine GDF5.

<10pg/ml

31.2pg/ml-2000pg/ml

Recognizes natural and recombinant Equine GDF5. There is no detectable cross-reactivity with other relevant proteins.

This Equine GDF5 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for Equine GDF5 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A376-R495) and test samples are added to the wells, a biotinylated detection specific for Equine GDF5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine GDF5 amount of sample captured in plate.

96-well plate precoated with anti-Equine GDF5 antibody, 1x plate Lyophilized recombinant Equine GDF5 standard, 2x10ng/tube Biotinylated anti-Equine GDF5 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.