Cell culture supernates, serum, plasma (Heparin or EDTA).

Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Rabbit GDF5.

<10pg/ml

31.2pg/ml-2000pg/ml

Recognizes natural and recombinant Rabbit GDF5. There is no detectable cross-reactivity with other relevant proteins.

This Rabbit GDF5 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for Rabbit GDF5 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A376-R495) and test samples are added to the wells, a biotinylated detection specific for Rabbit GDF5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit GDF5 amount of sample captured in plate.

96-well plate precoated with anti-Rabbit Gdf5 antibody, 1x plate Lyophilized recombinant Rabbit Gdf5 standard, 2x10ng/tube Biotinylated anti-Rabbit Gdf5 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.