Collapsin response mediator protein 2; CRMP2; Dihydropyrimidinase related protein-2; DPYL2; DPYSL2; DRP-2; N2A3; TOAD-64; Turned on after division, 64 kDa protein; ULIP 2 protein; ULIP2
The Phospho-CRMP2 (Thr514) BioAssay™ ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CRMP2 protein phosphorylation and expresssion profile in cells. It can be used for measuring the relative amounts of phosphorylated CRMP2 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on CRMP2 phosphorylation.
Sensitivity
>5000cells/well
Kit Components
472968A: Microtiter Strips, 2x96 wells 472968B: TBS, 10X, 1x24ml 472968C: Quenching Buffer, 1x24ml 472968D: Blocking Buffer, 1x50ml 472968E: Wash Buffer, 15X, 1x50ml *472968F: CRMP2 (Phospho-Thr514), 100X, 1x60ul *472968G: CRMP2 Pab, 100X. 1x60ul *472968H: GAPDH Mab, 100X, 1x60ul 472968J: IgG Pab (HRP), 2x6ml or 1x12ml 472968K: IgG Mab (HRP), 2x6ml or 1x12ml 472968L: Primary Antibody Diluent, 1x12ml 472968M: Substrate, 1x12ml 472968N: Stop Solution (2N Sulfuric Acid), 1x12ml 472968P: Crystal Violet Solution, 1x12ml *472968Q: SDS Solution, 1x24ml
Storage and Stability
Store all components at 4°C. Reconstituted *472968F, *472968G and *472968H are stable for 2 weeks at 4°C. *472968Q should be stored at RT or warmed up to RT if stored at 4ºC. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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