USBio Logo

R1660-01D Retinoblastom Antibody Sampler Kit, BioAssay™ (Rb)

Specifications
References
Brand
BioAssay™
Specificity
Detect endogenous levels of Rb protein only when phosphorylated at Ser780, Ser795, and Ser807/811, respectively.
Purity
Purified by Protein A and peptide affinity chromatography.
Gene ID
5925
Kit Type
Assay
Tests
4
Detection Method
Colorimetric/Fluorescent
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity
Detect endogenous levels of Rb protein only when phosphorylated at Ser780, Ser795, and Ser807/811, respectively.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Sherr, C.J. (1996) Science 274, 1672-1677. 2. Nevins, J.R. et al. (1992) Science 258, 424-429. 3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-790. 4. Hu, Q.J. et al. (1990) EMBO J. 9, 1147-1155. 5. Knudsen, E.S. and Wang, J.Y. (1997) Mol. Cell. Biol. 17, 5771-5783. 6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761. 7. Connell-Crowley, L. et al. (1997) Mol. Cell. Biol. 8, 287-301. 8. Kitagawa, M. et al. (1996) EMBO J. 15, 7060-7069. 9. Geng, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 194-199.
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved