Amies formulated a medium for transport of microorganisms using a balanced salt solution. Amies Broth with Charcoal is a non-nutritional, phosphate buffered type medium used to maintain the viability of microorganisms without a significant increase in growth. Charcoal was added to the formulation to neutralize the toxic effects of a fatty acids that are toxic to microorganisms. This medium is recommended for throat, vaginal, and wound samples.
Components shown as g/liter |
Calcium Chloride | 0.1 |
Charcoal | 10.0 |
Magnesium Chloride | 0.1 |
Potassium Chloride | 0.2 |
Monopotassium Phosphate | 2.5 |
Sodium Chloride | 3.0 |
Disodium Phosphate | 1.15 |
Sodium Thioglycollate | 1.0 |
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