APT Agar was formulated by Deibel, Evans and Niven when they were investigating thiamine requiring bacteria and heterofermentative lactobacilli. It is recommended for the microbiological examination of canned meat, poultry, sauerkraut and other food. APT Agar is also used in the microbiological assay of thiamine. The high metal content provide ions used in lactobacilli replication. Sorbitan Monooleate Complex (Tween 80) is a fatty acid source.

Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.

Lactobacillus fermentum (ATCC 9338): Growth Leuconostoc mesenteroides( ATCC 12291): Growth

Appearance:Light beige, homogeneous and free flowing

Solubility: Light to medium amber.and turbid to clear.

pH: 6.7 ± 0.2

Important Note: This product as supplied is intended for research use only, not forMedium for culturing Lactobacilli from meat products. A non-selective medium supporting growth of many other bacteria.

1. Dissolve 58.43grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.