Dextrose fermentation w/o pH indicator.
Description
Dextrose Broth is a basic medium used to study the fermentation of dextrose by microorganisms. It contains casein peptone used as carbon and nitrogen source and salt for electrolyte balance. Dextrose is the only fermentable carbohydrate. The medium does not contain a pH indicator.
Storage and Stabiity
Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Appearance
Light beige, homogeneous, free flowing powder.
Solubility
Pale to light yellow,, clear, complete.
Microbiological Analysis
Escherichia coli ATCC 25922: Growth Gas (+) Enterobacter aerogenes ATCC 13048: Growth Gas (+) Streptococcus pyogenes ATCC 19615: Growth Gas (-)
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions
1. Dissolve 20grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Components shown as g/liter |
Casein Digest of Peptone | 10.0 |
Dextrose | 5.0 |
Sodium Chloride | 5.0 |