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D9502 Drop-out Base With Galactose (DO, Dropout, Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Synthetic minimal media with galactose, Glucose free

Nitrogen, carbon, trace element, and vitamin source for yeast growth. Supplement with the appropriate DO Mix for selection and propagation of yeast transformants. Drop-out Base With Galactose is specially formulated to be glucose free for the rapid induction of galactose-inducible genes.

All lots are quality control tested using the appropriate yeast strains and transformants.
• Convenient Powders • Useful for One-Hybrid and Two-Hybrid Systems • Competitively Priced...!
BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous: Proprietary blending technology ensures complete mixing of components
Appearance
White, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
pH 4.0-5.0
Directions per Liter
Dissolve 26.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Galactose 20
YNB (w/o amino acids w/ ammonium sulfate) 6.7
Total26.7g/L
100g makes 3.75 liters
References
Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A:210, CSHL Press (1994)
USBio References
No references available
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