Schaedler Broth was first described by Schaedler et al. as an enriched medium for the isolation of anaerobes from mixed bacteria flora from the gastrointestinal track of mice. Mata et al., substituting Trypto Soy Broth for casein digest peptone in the Schaedler formulation, found that this medium gave excellent recovery of anaerobic and microaerophilic organisms from human fecal and gastrointestinal specimens. Schaedler Broth gave the highest rate of growth and response of anaerobic bacteria of nine broth media studied by Stalons et al.. General growth requirements are supplied by Trypto Soy Broth, dipeptone, yeast extract and dextrose. Hemin and cystine are added for specific requirements of some anaerobes. Blood and other enrichments may be added to enhance growth characteristics.