Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates.
| Components shown as g/liter |
| Potassium phosphate dibasic | 9.4 |
| Potassium phosphate monobasic | 2.2 |
| Tryptone | 12 |
| Yeast Extract | 24 |
| Total: | 47.6g/Liter |
| Add 4ml glycerol/liter final solution. |
| 500g makes 10.5 liters |
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