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Axol Human Neural Progenitor Cells
(hNPCs)

Bottle and ManualAxol hNPCs are derived from integration-free, induced pluripotent stem (iPS) cells under fully defined neural induction conditions.

The hNPCs express typical markers of cerebral cortical neural stem and progenitor cells e.g. Pax6 and Foxg1, and spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture (see below). Additionally, Axol hNPCs are capable of generating a spectrum of cortical neurons that are electrically active and have the ability to form functional synapses and circuits in vitro

Cerebral cortical neurons are implicated in numerous neurological diseases including Alzheimer’s disease, autism, epilepsy and stroke. Despite strong interest in these diseases, high quality human in vitro cell models for cortical neurophysiology were unavailable until now. With Axol hNPCs, we provide neuroscience researchers with a high quality in vitro human system to complement their existing studies of cortical development, function and diseases using animal models.

 

 

2 days after thawing

7 days after thawing

21 days after thawing

Click images to enlarge

 

Cat #

Product Name

Gender

Donor Age

Starting Material

Reprogramming Method

Neural Induction Method

Genetic Mod.

Size

ax0011 hNPC
Donor A
Male New Born Cord Blood CD34+ Cells Episomal Plasmid Monolayer
Chem-defined medium
No 2M
cells
ax0013 hNPC
Donor C
Male New Born Cord Blood CD34+ Cells Episomal Plasmid Monolayer
Chem-defined medium
No 2M
cells
ax0014 hNPC
Donor D
Female New Born Cord Blood CD34+ Cells Episomal Plasmid Monolayer
Chem-defined medium
No 2M
cells
ax0015 hNPC
Donor E
Male New Born Cord Blood CD34+ Cells Episomal Plasmid Monolayer
Chem-defined medium
No 2M
cells
ax0016 hNPC
Donor F
Female New Born Cord Blood CD34+ Cells Episomal Plasmid Monolayer
Chem-defined medium
No 2M
cells

 


Advantages of Axol hNPCs

Specifications

Applications

  • Human cells
  • Available in industrial quantity
  • High purity neural progenitor population
  • Ready-made and easy to differentiate
  • Robust enough to undergo genetic manipulation
  • Supplied with extensive functional and molecular characterization data
  • Capable of differentiating to a spectrum of cortical excitatory and inhibitory neurons
  • Remain viable in culture for months to facilitate reproducible and long term studies
  • Cells from multiple donors are available for purchase allowing data comparison among biological replicates
  • Quantity: 2 million expandable cells per vial
  • Presentation: Cells are provided cryopreserved in 1 ml of freezing medium
  • Storage Condition: Liquid nitrogen
  • Growth Properties: Adherent 
  • Studying Corticogenesis in vitro
  • Studying neural network formation and signal transmission 
  • Disease modelling
  • Neurotoxicity testing
  • Preclinical drug efficacy testing
  • Research and development of cell therapies in animal models

 

 

One vial of Axol hNPCs (2 million cells) can give rise to two 6-well plates of mature functional neurons at 70% confluence (12 million cells).