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New BioAssay™ LPS ELISA,
Finally… a Substitute for the LAL Test

Lipopolysaccharides (LPS), also known as endotoxin, are one of the main components of Gram-negative bacteria. LPS can cause fever, shock and death. With the growing popularity of genetic engineering techniques in biological products and reagents, LPS has become an important indicator to monitor both residual bacteria and pyrogenicity in biological products.


 

 

 

 

 

 

LPS, a thymus independent antigen (TI antigen), cannot induce antibody affinity maturation nor immunological memory. Because of this, it is very difficult, if not impossible, to obtain high-affinity antibodies by using conventional and routine immunological methods. Thus, LPS antibodies and related immunoassay kits are quite rare in the current market.

Using recently developed technology that greatly enhance LPS immunogenicity, we are now able to produce monoclonal antibodies with high specificity towards LPS. We have incorporated such an antibody into the LPS Detection Kit (Catalog # 026552) which is low cost, easy to use, and has high throughput capacity, enabling analysis of 88 samples per assay.

The following table compares the LPS ELISA detection method with the traditional LAL (Limulus Amebocyte Lysate) test detection method:


Comparison

LAL Agglutination (USP Standard)

LPS ELISA KIT

Principle
Agglutination Reaction: LAL reacts with Endotoxin Specific antigen-antibody interaction
Result Quantification
Semi-quantitative Quantitative
Data Reading
Visual Microplate reader
Sample Quantity
1 Sample At A Time 88 samples per run
Vector
Test Tube 96 well plate
Operation and Calculation
Multiple dilution, complex calculation Certain dilution, simple calculation
Sample Volume
>100ul 50ul
Pricing
Higher Cost Lower Cost

 

The structure of LPS is illustrated above. Bacterial LPS typically consists of a hydrophobic domain known as Lipid A (or endotoxin), a non-repeating “core”oligosaccharide, and a distal polysaccharide (or O-antigen). The anti-LPS antibody in the LPS ELISA Kit is directed towards the conserved region of LPS and could thus measure LPS originating from different bacteria, resulting in a versatile kit with a wider range of application.