Electrophoresis Grade Agarose is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. Low EEO Agarose is recommended for a wide array of molecular biology procedures and offers enhanced resolution ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
- Analytical DNA Gels ≥ 1000bp
- Blotting Assays (Southern, Northern)
- Radial Immunodiffusion
- Restriction Enzyme digestion products
- For use with all buffer systems
A1015 is the workhorse Agarose gel of the US Biological products. It has the best performance characteristics for the widest variety of DNA applications. It provides the best resolution and lowest melting point of the Agarose products.
A1020 is suitable for protein and large DNA separations ≥1000bp. The low melting temperature allows for the recovery, without damage, of nucleic acids below their denaturing temperatures.
A1025, High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications.
A1045, Extra High EEO Agarose is especially suitable for large molecule applications due to the exceptionally high cathode migration possible.
Agarose DNA Fragment products (A1000, A1005, A1010, A1012) are manufactured specifically for small primer size fragments in the range of 10-2000 base pairs and they exhibit exceptionally high transparency even at high concentrations (5%).
United States Biological Agarose products
Gel point (1.5%)
|A1000||Agarose DNA Fragments 10-500bp||≤ 75°C (3%)||24-28°C (3%)||≥ 200g/cm2
|A1005||Agarose DNA Fragments 50-1000bp||≤ 75°C (3%)||≤ 35°C (3%)||≥ 750g/cm2||≤ 0.13|
|A1010||Agarose DNA Fragments 150-1500bp||≤ 89ºC (4%)||≤ 40°C (4%)||≥ 2000g/cm2||≤ 0.12|
|A1012||Agarose DNA Fragments 50-2000bp (3:1 Blend)||≤ 93°C||≤ 40.5°C||≥ 2000g/cm2||≤ 0.12|
|A1015||Agarose Low EEO (MB)||86.5-89.5°C||34.5-37.5°C||≥ 2500g/cm2||0.09-0.13|
|A1016||Agarose Low EEO (EP)||86.5-89.5°C||34.5-37.5°C||≥ 2500g/cm2||0.09-0.13|
|A1020||Agarose Low Melting||≤ 66°C||24-28°C||≥ 500g/cm2||≤ 0.12|
|A1025||Agarose High Gel Temp||85.5-88.5°C||39.5-42.5°C||≥ 1200g/cm2||≤ 0.14|
|A1030||Agarose High Gel Strength||86-90°C||34.5-37.5°C||≥ 3000g/cm2||≤ 0.135|
|A1035||Agarose Med EEO||86.5-89.5°C||34.5-37.5°C||≥ 2000g/cm2||0.16-0.19|
|A1040||Agarose High EEO||86.5-89.5°C||34.5-37.5°C||≥ 1000g/cm2||0.23-0.26|
|A1045||Agarose Extra High EEO||86.5-89.5°C||34.5-37.5°C||≥ 1100g/cm2||≥ 0.30|