|Agarose DNA Fragments 10-500bp||A1000|
|Agarose DNA Fragments 50-1000bp||A1005|
|Agarose DNA Fragments 150-1500bp||A1010|
|Agarose DNA Fragments 50-2000bp (3:1 Blend)||A1012|
|Agarose Low EEO (MB)||A1015|
|Agarose Low EEO (EP)||A1016|
|Agarose Low Melting||A1020|
|Agarose High Gel Temp||A1025|
|Agarose High Gel Strength||A1030|
|Agarose Med EEO||A1035|
|Agarose High EEO||A1040|
|Agarose Extra High EEO||A1045|
1. Recommendations for dissolving Agarose The normal way to dissolve agarose is by microwaving for 2 minutes on High. We recommend opening the microwave at 1 minute and swirling the flask very gently, with care being taken not to boil over the liquid. Replace flask in the microwave and continue for the remaining 1 minute. This procedure applies to all regular agaroses including the derivatized ones (A1005, A1020 etc.) except for A1000 which is very viscous. A1000 agarose may take 3 minutes or more, swirling at 1 and 2 minutes.
All agaroses can be autoclaved at 121°C for 15 minutes. This procedure is great for all agaroses, especially A1000. However, some labs don't have autoclaves, and therefore the use of a microwave or a heated waterbath is the preferred choice.
2. What does EEO stands for? Are there any differences between low, medium and high EEO?
EEO means "electroendoosmosis," which is a process that occurs during preparative protein electrophoresis. The agarose gel matrix contains fixed anionic charges (-'s) which cannot move within the electrical field. As electrophoresis proceeds, dissociable cations (+'s) can migrate toward the cathode, dragging water along with them (the hydration shell which surrounds all ions in solution). This leads to swelling and distortion of the gel. In addition the movement of water in the gel is in a direction opposite to the movement of the proteins, which leads to unwanted broadening of the protein band. Low and Medium EEO Agarose is typically used for separation of DNA fragments. High EEO Agarose is recommended for the electrophoresis of serum proteins and immunoelectrophoresis
The extent to which this process occurs can be altered by changing the porosity of the agarose, depending on the molecular weight ranges one is trying to separate, and other chemical factors. High EEO is generally undesirable, but sometime unavoidable when separating very high molecular weight proteins. Researchers often try to find the lowest EEO agarose product that meets their needs.