Z-TECT™ High Sensitivity ELISA Secondary Kits and Reagents
Z-TECT™ High Sensitivity ELISA Secondary MonsterHRP™ Products
While the user only needs to substitute the secondary reagent in an established ELISA assay, the large signal amplification resulting from the high number of HRP molecules provided by Z-TECT™’s technology, often requires to calibrate and “tone down” the concentrations of capture and/or detection antibodies. Furthermore, the various buffers provided in the Akrivis kits were formulated to ensure optimal performance of its Z-TECT™ HS ELISA Secondary MonsterHRP™ Products. Therefore Akrivis offers three options for each of its Z-TECT™ HS ELISA Secondary MonsterHRP™ Products:
- 1-Plate Trial Kit that contains all reagents and materials necessary (96x-microwell plate, adhesive plate sealers, buffers and detection reagents) for a quick evaluation via indirect, sandwich or competitive ELISA;
- 5-Plate Optimization Kit that contains all reagents and materials necessary (96x-microwell plates, adhesive plate sealers, buffers and detection reagents) for a more thorough evaluation of Akrivis reagents and buffers via indirect, sandwich or competitive ELISA. In order to expedite the optimization process, Akrivis has developed a SMARTPLATE™ protocol design that consists of a pre-designed [4x4x4] concentration array of the capture antibody (for sandwich assays), detection antibody and secondary reagent to minimize Non-Specific Background and optimize Signal:Noise Ratio. The 5-Plate Optimization Kit also provides the user with the opportunity to screen and compare various Akrivis buffers and detection reagents included in the kits;
- Standalone Reagents and Buffers that allow the user, once optimization is complete, to purchase separately the Z-TECT™ HS ELISA Secondary MonsterHRP™. However, for optimal performance, it is highly recommended to use it in conjunction with Akrivis’ Z-TECT™ Diluent and Wash Buffer.
- 5-Plate Ancillary Kits that contain all materials (full 96-well plates and adhesive plate sealers), buffers (coating, blocking, dilution and wash), diluents (MonsterHRP™ Diluent) and signal reagents (HRP substrate and stop solution), EXCEPT the Z-TECT™ HS ELISA Secondary MonsterHRP™ Reagent, sufficient for 5 full 96-well plates.
Z-TECT™ HS ELISA Secondary MonsterHRP™ Kits contain all the reagents and materials necessary for performing an indirect, sandwich or competitive ELISA assay (96-microwell plates, adhesive plate sealers, buffers and detection reagents). Microwell plates can be either full plates (96x) or strip plates (12x 8-strips). All reagents and materials are fully compatible with standard lab research equipment (pipettes, incubators, microwell plate washers and readers).
|AK-AAXA-AA||5-Plate Ancillary Kit with all materials, buffers, diluents and detection reagents, EXCEPT MonsterHRP™ reagent, for 5 full 96-well or 8x12-strip plates||full or strip plates|
|BUF-DB||Akrivis MonsterHRP™ Diluent (1X)||250ml or 1L|
|BUF-WA||Akrivis MonsterHRP™ Wash Buffer (20X)||250ml or 1L|
|BUF-DA||Akrivis Dilution Buffer (5X)||250ml or 1L|
|BUF-CA||Akrivis Coating Buffer (5X)||250ml or 1L|
|BUF-BA||Akrivis Blocking Buffer (5X)||250ml or 1L|
|SUB-AA||Akrivis HRP TMB Substrate (1X)||250ml or 1L|
|STP-AA||Akrivis TMB Stop Solution (1X)||250ml or 1L|