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United States Biological
April 2009 Issue 1| Subscribe                                                                                               www.usbio.net
Newsletter


Breast tumorEverything for Drosophila Studies:

  Our new Drosophila K12 medium is continuing to be a great success with Fly labs. Try a free sample this month and see what it can do for yours. Compared to traditional rich media containing molasses or corn, K12 yields more larvae and adult flies; increases the level of egg deposition; and increases the overall hatching rate. Of course we also have great prices on other drosophila lab supplies such as Tegosept anti-fungal agent, food-grade Drosophila Agar, and highly-purified recombinant Drosophila GAGA-POZ peptide.



Drosophila Egg

Affinity Purified Antibodies to Drosophila:




Cat # Antibody to: Type: Apps:
G8170-47 Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) Mab B
G8170-40 Glycogen Synthase Kinase 3 (GSK3) Mab B
B2179 BMAL1 (MOP3) Pab E, B
T8666-31 TRPL (Transient Receptor Potential-like Protein) Pab B, IH
A1125-12A Akt, phosphorylated (Ser505) Pab B, IC
M4590-04A MOP3 (Members of PAS Superfamily, BMAL1) Pab E, B
C7942-10 Cryptochrome (CRY) Pab E, B
P1001-37R p70 S6 Kinase, phosphorylated, Thr398 Pab B
D1499-06A DcR1 (TRAIL-R3) Pab B
D3520-06 Dicer 2 Pab E,B
P3367-50B Phantom (Exo, Exocephalon) Pab B, IP, IC



This month's Special:
Free 1L sample of Drosophila Diet Medium K12

  K12 Medium is a new insect food particularly indicated for Drosophila melanogaster flies' growth and propagation. Our medium has advantages over existing rich traditional media (containing molasses or corn):

Hatching Time   -Greatly improves (about 100%) the overall hatching rate (18-20 hours). Flies raised on K12 Medium hatch over a 4-5 day period instead of the 2-3 days of traditional media, greatly facilitating the collection of virgins and the use of young adults for healthy crosses (Fig.1).

  -Yields about 200% more larvae and adult flies. This is particularly relevant for the propagation of precious mutant Drosophila strains, which are less viable and used as models for studying human genetic diseases.

  -Increases the levels of egg deposition (18-20 hours) by about 50%.


Drosophila Growth Media
A New Choice

  The common fruit fly, Drosophila melanogaster, has been used as a model organism in research for nearly a century. Today, Drosophila are used to study topics such as developmental biology, genetics, and as models for human protein systems and diseases. Drosophila stocks are grown and propagated on media that typically contain agar, corn flour, sugar, in the form of sucrose, dextrose or molasses, and yeast.  The goal of such media is to propagate healthy stocks and to generate an ample number of offspring with a well-defined life cycle.

"Time flies like an arrow; fruit flies like a banana."
-Groucho Marx


  D. melanogaster exhibits complete metamorphism, meaning the life cycle includes an egg, larval form, pupa and finally emergence (eclosure) as a flying adult. The larval stage has three instars, or molts.
fruit fly; www.flickr.com/photos/max_westby/54275159/Typical life cycle by day:
Day 0: Female lays eggs
Day 1: Eggs hatch
Day 2: First instar (one day in length)
Day 3: Second instar (one day in length)
Day 5: Third and final instar (two days in length)
Day 7: Larvae begin roaming stage. Pupariation (pupal formation) occurs 120 hours after egg laying
Day 11-12: Eclosion (adults emerge from the pupa case). Females become sexually mature 8-10 hours after eclosion.
The time from egg to adult is temperature-dependent. The above cycle is for a temperature range of 21-23°C. The higher the temperature, the faster the generation time.

  Females can lay up to 100 eggs/day. Virgin females are able to lay eggs, however they will be sterile and few in number. Females remain virgins for only 8-10 hours after eclosure and must be collected within this time frame.

  Fruit flies are maintained as live culture by transferring adults to fresh medium every few generations. Therefore, good quality food yielding high numbers of healthy flies is critical for stock maintenance. K12 medium has certain advantages over existing rich traditional media (containing molasses or corn).

  It greatly improves (about 100%) the overall hatching rate (18-20 hours). Flies raised on K12 Medium hatch over a 4-5 day period instead of the 2-3 days of traditional media, greatly facilitating the collection of virgins and the use of young adults for healthy crosses. Fly yields average about 200% more larvae and adult flies. This is particularly relevant for the propagation of precious mutant Drosophila strains, which are less viable and used as models for studying human genetic diseases. Levels of egg deposition (18-20 hours) are increased by about 50%.

  K12 Medium is resistant to microbial contamination and to liquification. This latter feature is particularly relevant for successful maintenance of sick stocks, quick expansion of stocks  and for mass culture of larvae and adults. After nutritional and developmental studies conducted in several wild type and mutant stocks of D. melanogaster, this medium was developed. It contains the same nutritional sources found in commonly used fly food plus K12, an additional natural ingredient that improves fly development by increasing the number of healthy hatching adults per cross. K12 is extracted from natural sources, currently used in human and animal food industry, and is neither toxic nor cancerous.

  Other US Biological Drosophila media:
-D9600-07, Drosophila Diet Medium (Powder) (Caltech Medium, Fruitfly diet)

-D9600, Drosophila D-22 Insect Medium (Powder)
Cell Culture medium used for the maintenance of Drosophila melanogaster and other insects.


Online Databases:
Flybase (http://flybase.org/)
The Interactive Fly (http://www.sdbonline.org/fly/aimain/1aahome.htm).
FlyNome (flynome.come)
Homophila (superfly.ucsd.edu/homophila)

Colon Tissue
Clock Protein

Antibodies to Drosophila Clock Proteins :
CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

Clock
Rabbit Pab
Immunogen: A 17aa C-terminus peptide of Drosophila Clock. Suitable for use in ELISA and Western Blot.
(Ammonium Sulfate Purified)

Period Clock Protein 1
Rabbit Pab.
Immunogen: A 21aa C-terminus peptide of Drosophila Clock. Suitable for use in ELISA, Immunohistochemistry, and Western Blot. (Ammonium Sulfate Purified)

Clock (dClock)
Rabbit Pab.
Immunogen: A 17-aa peptide sequence within the c-terminus of dClock. Drosophila sequence has no significant sequence homology with mammalian Clock or Per proteins.Suitable for use in ELISA and Western Blot.
( Serum)

Period Clock Protein (dPER)
Rabbit Pab.
Immunogen: A 21aa C-terminus peptide of Drosophila Clock. Suitable for use in ELISA and Western Blot. (Serum)


USBio's 2009 catalog is ready for shipping.
US Bio CatalogIf you haven't reserved your copy yet, you can do it now . It features an expanded reference section and over 20,000 new products.
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