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AUGUST 2011
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NEW Stemcell Antibodies
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Mesenchymal Stem Cells
Mesenchymal stem cells or MSCs are originally name as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, B-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis.
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Neural Progenitor Cells
Neural progenitor cells are a population of cells with self-renewal and multipotency for differentiation into astrocytes, neurons, and oligodendrocytes. The NPCs have large therapeutic value for the diseases of nervous system.
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![Immunofluorescence of purified antibody to NUP43 on HeLa cell. [antibody concentration 10 ug/ml]](../images/immunofluorescentAB.jpg)
Immunofluorescence of purified antibody to NUP43 on HeLa cell. [antibody concentration 10 ug/ml]
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SPECIAL Biological Buffers
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Common Buffer Table (at 20°C)
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Buffer
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pKa
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pH range
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MW
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HEPES
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7.55
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6.8-8.2
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238.3
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MOPS
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7.28
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6.5-7.9
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209.3
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MES
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6.15
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5.8-6.5
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195.2
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PIPES
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6.8
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6.1-7.5
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302.4
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TAPS
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8.49
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7.7-9.1
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243.3
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Tricine
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8.15
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7.4-8.8
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179.2
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Tris
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8.2
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7.5-9.0
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121.1
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Choosing the right buffer
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Biological reactions depend on the pH of the solution being kept within a specific range. This is accomplished with buffers which maintain the required pH parameters. Buffers are an everyday but crucial part of your lab supplies. You go through a lot of them and need to be sure that they are top-quality.
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United States Biological sells a wide variety of premium buffers and buffer components, and since we are using the same prices that have been in effect since 1995 for most of our biochemicals, you can afford to stock up.
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FEATURED Aquaporin
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Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
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ARTICLE Biological Lasers
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In a new study published in Nature Photonics a single cell biological laser has been developed by using human embryonic kidney cells transfected with Green fluorescent protein (GFP) from the jellyfish, Aequorea victoria. The three basic components of a laser are:
Pulsed lasing image of GFP transfected human cell.
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a lasing material (such as a crystal, gas, semiconductor, dye, etc.),
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a pump source (which adds energy to the lasing material, e.g. flash lamp, electrical current, radiation from a laser, etc.), and
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an optical cavity consisting of reflectors to act as the feedback mechanism for light amplification.
In this case, the lasing material comprises the overexpressed GFP molecule inside the cell matrix. The pump source was a pulsed blue laser and the optical cavity was a single cell placed between two highly reflecting mirrors spaced 20 um apart.
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