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NGAL (Lipocalin 2)

HNL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor p25, alpha-2 microglobulin-related subunit of MMP-9

 

 

Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25kD glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury.

Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including alpha1-microglobulin and beta-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke. Human neutrophil gelatinase-associated lipocalin (NGAL), and its mouse analog 24p3, are members of the lipocalin family of small secreted proteins. These proteins are up-regulated in a number of pathological conditions, including cancers, and may function

NGAL (Lipocalin 2) Antibodies

Cat #

Product Name

Type

Host

Source

Clone #

167733 NGAL Mab Mouse Porcine 28
Recognizes porcine NGAL.
L2499-14A NGAL Mab Mouse Human 13A128
Recognizes human NGAL. Does not cross-react with NGAL from other mammalian species tested.
L2499-14C NGAL Mab Mouse Human 13A129
Recognizes human NGAL. Does not cross-react with NGAL from other mammalian species tested.
L2499-14D NGAL Mab Mouse Human 13A130
Recognizes human NGAL. Does not cross-react with NGAL from other mammalian species tested.
L2499-14E NGAL Mab Rat Mouse 13A131
Recognizes mouse NGAL.
L2499-14F NGAL Mab Rat Mouse 13A132
Recognizes mouse NGAL.
L2499-14G NGAL Mab Mouse Canine 13A133
Recognizes canine NGAL.
L2499-14H NGAL Mab Mouse Canine 13A134
Recognizes canine NGAL.
L2499-14M NGAL (Biotin) Mab Rat Mouse 13A135
Recognizes mouse NGAL.
L2499-14N NGAL (Biotin) Mab Mouse Canine 13A136
Recognizes canine NGAL.
L2499-15 NGAL Mab Mouse Human 6F154
Recognizes human Lipocalin 2. Epitope specificity differs from that of clone 6F155. Does not crossreact with Lipocalin 2 from other species.
L2499-15C NGAL (Biotin) Mab Mouse Human 6F154
Recognizes human NGAL.
L2499-16 NGAL Mab Mouse Human 6F155
Recognizes human Lipocalin 2. Epitope specificity differs from that of clone 6F154. Species Crossreactivity: Cynomolgus monkey (Macaca fascicularis)
L2499-17B NGAL Mab Mouse Rat 7F2
Specific for rat NGAL and shows no crossreaction with human NGAL.
L2499-17C NGAL Mab Mouse Rat 7F3
Recognizes rat NGAL. Does not crossreact with human NGAL.
L2499-17D NGAL(Biotin) Mab Mouse Rat 7F1
Recognizes rat NGAL. Does not crossreact with human NGAL.
L2499-17E NGAL Mab Mouse Human 8D2
Binds cynomolgus monkey (Macaca fascicularis) NGAL and cross-reacts with human NGAL. Binds human NGAL in complex with MMP-9.
L2499-17G NGAL Mab Mouse Porcine 048-17
Binds porcine NGAL

Recombinant NGAL Antigens

Source: Recombinant protein, expressed in E.coli, corresponding to full length amino acid sequence of NGAL, purified and produced without any tags.

Cat #

Product Name

Source Antigen

168622 Lipocalin 2, Recombinant Recombinant Human from E. coli
168623 Lipocalin 2, Recombinant Recombinant Mouse from E. coli
168624 Lipocalin 2, Recombinant Recombinant Rat from E. coli
168625 Lipocalin 2, Recombinant Recombinant Canine from E. coli
168626 Lipocalin 2, Recombinant Recombinant Porcine from E. coli
168627 Lipocalin 2, Recombinant Recombinant Monkey from E. coli

NGAL ELISA Kits (96 Tests)

Neutrophil Gelatinase Associated Lipocalin (NGAL) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NGAL in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Cat #

Product Name

Detection Range

Sensitivity

027143 Neutrophil Gelatinase Associated Lipocalin (NGAL) BioAssay™ ELISA Kit (Human) 31.2-2000pg/ml 13.5pg/ml
027144 Neutrophil Gelatinase Associated Lipocalin (NGAL) BioAssay™ ELISA Kit (Rat) 6.25-400pg/ml 2.84pg/ml
027145 Neutrophil Gelatinase Associated Lipocalin (NGAL) BioAssay™ ELISA Kit (Mouse) 0.156-10ng/ml 0.066ng/ml
027146 Neutrophil Gelatinase Associated Lipocalin (NGAL) BioAssay™ ELISA Kit (Porcine) 1.56-100ng/ml 0.57ng/ml

 

References:

     
  1. Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003). J Am Soc Nephrol 14:2534-43.
  2.   
  3. Identification of neutrophil gelatinase-associated lipocalin (NGAL) as a discriminatory marker of the hepatocyte-secreted protein. Kjeldsen L et al (1993). Biotechnol Bioeng 2005 Aug 20;91(4):502-15
  4.   
  5. Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils, Kjeldsen L et al (1996). J Immunol Meth 198:155-64.
  6.   
  7. Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 2005 Apr 2-8;365(9466):1231-8
  8.   
  9. Cloning and identification of 5'-untranslated region (UTR) and 3'-untranslated region of neutrophil gelatinase-associated lipocalin (NGAL) gene from esophageal carcinoma cell line SHEEC. Ai Zheng 2003 Feb;22(2):143-7
  10.   
  11. The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition. Mol Cell 2002 Nov;10(5):1033-43
  12.   
  13. Clinical manifestations of atherosclerosis in an elderly population are related to plasma neopterin, NGAL and endothelin-1, but not to Chlamydia pneumoniae serology. Int Angiol 2002 Jun;21(2):173-9
  14.   
  15. The high molecular weight urinary matrix metalloproteinase (MMP) activity is a complex of gelatinase B/MMP-9 and neutrophil gelatinase-associated lipocalin (NGAL). Modulation of MMP-9 activity by NGAL. J Biol Chem 2001 Oct 5;276(40):37258-65
  16.    
  17. The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition. Mol Cell 2002 Nov;10(5):1033-43.
  18.   
  19. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996). Gut 38:414-420.
  20.   
  21. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV (2001). J Am Soc Nephrol 12:787A.
  22.   
  23. Kjeldsen L, Koch C, Arnljots K, Borregaard N (1996). J Immunol Methods 198:155-164.
  24.   
  25. Tong Z, Kunnumakkara AB, Wang H, Matsuo Y, Diagaradjane P, Harikumar KB, Ramachandran V, Sung B, Chakraborty A, Bresalier RS, Logsdon,C, Aggarwal BB, Krishnan S, Guha S (2008). Canc Res 68: 6100-6108.
  26.   
  27. Lipocalin 2 antagonizes the proangiogenic action of ras in transformed cells. Venkatesha S, Hanai J, Seth P, et al. Mol Cancer Res. 2006. Nov;4(11):821-9. PMID: 17114340.
  28.   
  29. Cell specific markers for eosinophils and neutrophils in sputum and bronchoalveolar lavage fluid of patients with respiratory conditions and healthy subjects. Metso, T et al; Thorax 2002, 57: 449
  30.   
  31. Hraba-Renevey S, Türler H, Kress M, Salomon C, Weil R (1989). SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism. Oncogene 4:601-608.
  32.    
  33. Mouse oncogene protein 24p3 is a member of the lipocalin protein family. Flower DR, North AC, Attwood TK (1991). Biochem Biophys Res Commun 180:69-74.
  34.   
  35. Identification of a new acute phase protein. Liu Q, Nilsen-Hamilton M (1995). J Biol Chem 270:22565-22570.
  36.   
  37. High expression in involuting reproductive tissues of uterocalin/24p3, a lipocalin and acute phase protein. Ryon J, Bendickson L, Nilsen-Hamilton M (2002). Biochem J 367:271-277.
  38.   
  39. Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem. 268:10425-32.
  40.   
  41. Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 2005, Apr 2-8;365(9466):1231-8