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Zymolyasezymolyase yeast

Zymolyase, produced by a submerged culture of Arthrobacter luteus, is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cell walls in this preparation is b-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases specifically laminaripentaose specifically as the main and minimum product unit. This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition. Further information related to Zymolyase is obtained in the references below.

 

Zymolyase Protocol

Catalog #

Product Name

Z1000 Zymolyase 20T (Lyticase, Yeast Lytic Enzyme)
Z1001 Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)
Z1004 Zymolyase 100T (Lyticase, Yeast Lytic Enzyme)
Z1005 Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)

 

US Biological application references:
1. Kim, S.K. et al., (2003) Biotechnology Letters 25: 1769-1774.
2. Harsay, E. and Schekman, R. (2007) Mol. Biol. Cell. 18:1203-1219.
3. Deloche, O. and Schekman, R.W. (2002) Mol. Biol. Cell 13:4296-4307.
4. Gardocki, M.E. and Lopes, J.M. (2003) J. Biol. Chem. 278:38646-38652.
5. Deloche, O. et al., (2001) Mol. Biol. Cell 12:475-485.
6. Ritch, J.J. et al., (2010) FEMS Yeast Res. 10:168-176.
7. Amiott, E.A. et al., (2009) Mol Biol. Cell 20:5026-5035.
8. Ganguly, S. et al., (2009) BBRC 387:661-665.
9. Quimby, B.B. et al., (2000) Mol. Biol. Cell 11:2617-2629.
10. Larsen, L.S.Z. et al., (2007) J. Virology 81:6957-6972.
11. Checkley, M.A. et al., (2010) Molecular and Cellular Biology. 30: 382-398.