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137167 POU6F1, Recombinant, Human, aa1-301, His-Tag (POU Domain Class 6 Transcription Factor 1, BRN5, MPOU, TCFB1) CAS:

Specifications
References
Grade
Purified
Molecular Weight
35
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

POU6F1 belongs to the POU transcription factor family. This protein is transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). In the embryo, it is expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.

Source
Recombinant protein corresponding to human POU6F1, fused to His-tag at N- terminal expressed in E.coli.
Molecular Weight
~35kD (324aa) (MALDI-TOF)
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPGISSQ ILTNAQGQVI GTLPWVVNSA SVAAPAPAQS LQVQAVTPQL LLNAQGQVIA TLASSPLPPP VAVRKPSTPE SPAKSEVQPI QPTPTVPQPA VVIASPAPAA KPSASAPIPI TCSETPTVSQ LVSKPHTPSL DEDGINLEEI REFAKNFKIR RLSLGLTQTQ VGQALTATEG PAYSQSAICR FEKLDITPKS AQKLKPVLEK WLNEAELRNQ EGQQNLMEFV GGEPSKKRKR RTSFTPQAIE ALNAYFEKNP LPTGQEITEI AKELNYDREV VRVWFCNRRQ TLKNTSKLNV FQIP
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥85% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 20% glycerol, 1mM DTT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Wey E, Lyons GE, et al: Eur J Biochem. 1994, 220(3):753-62. 2. Messier H, Brickner H, et al: Mol Cell Biol. 1993, 13(9):5450-60.
USBio References
No references available
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