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137345 PDE4D7, Active, Recombinant, Human, GST-Tag (PDE4D, cAMP-specific 3',5'-cyclic Phosphodiesterase 4D, DPDE3, PDE43, DPDE3) CAS:

Specifications
References
Grade
Purified
Swiss Prot
Q08499
Accession Number
NM_001165899
Molecular Weight
111
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

PDE4D7 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Source
Recombinant corresponding to aa2-748 from human PDE4D7, fused to GST-tag at N-terminal, expressed in a Baculovirus infected Sf9 cell expression system.
Molecular Weight
~111kD
Applications
Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Specific Activity
~8000pmol/min/ug
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Sf9 insect cells
Purity
Purified (~65%)
Concentration
As reported
Form
Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 3mM DTT, 20mM glutathione, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Wang, D., et al., (2003). Cell Signal. 15(9):883-91. 2. Houslay, M.D. (2005). Mol Pharmacol. (2005). 68(3):563-7. 3. Zhang, K.Y.J., et al., Mol. Cell 15: 279-286 (2004). 4. Card, G.L., et al., Structure 12: 2233-2247 (2004).
USBio References
No references available
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