Recombinant Rat from E. coli

O35112

Trp28~Lys527 (Accession No: O35112)

MGHHHHHHSGSEF-WYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI NNAKISDEKR FVCMLVTEDN VFEAPTLVKV FKQPSKPEIV NRAAFLETEQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV DGEVSILFKK EIDPGTQLYT MTSSLEYKTT KSDIQMPFTC SVTYYGPSGQ KTIYSEQAIF DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQAE GIRSSNTYTL TDVRRNATGD YKCSLIDQRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIQWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADDISDENRE KVNDQAK

N-terminal His-Tag

57.6kD

Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Recombinant, E. coli

Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.