Recombinant protien corresponding to aa63-264 with an N-Terminal His Tag, expressed in E. coli. UniProt/Accession: P22734
Predicted Isoelectric Point
5.4
Predicted Molecular Weight
23.8kD
Accurate Molecular Weight
24kD by SDS-PAGE under reducing conditions
Subcellular Location
Membrane, Extracellular Matrix
Amino Acid Sequence
GDPQSVLE AIDTYCTQKE WAMNVGDAKG QIMDAVIREY SPSLVLELGA YCGYSAVRMA RLLQPGARLL TMEMNPDYAA ITQQMLNFAG LQDKVTILNG ASQDLIPQLK KKYDVDTLDM VFLDHWKDRY LPDTLLLEKC GLLRKGTVLL ADNVIVPGTP DFLAYVRGSS SFECTHYSSY LEYMKVVDGL EKAIYQGPSS PDKS
Applications
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.