Recombinant protein corresponding to aa171-431 with two N-Terminal Tags, His-Tag and T-7Tag from human PROM1 expressed in E. coli (O43490)
Predicted Molecular Weight
32.8kD
Predicted Isoelectric Point
7.9
Subcellular Location
Cell projection, cilium, photoreceptor outer segment. Apical cell membrane; Multi-pass membrane protein
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM IGIFYGFVAN HQVRTRIKRS RKLADSNFKD LRTLLNETPE QIKYILAQYN TTKDKAFTDL NSINSVLGGG ILDRLRPNII PVLDEIKSMA TAIKETKEAL ENMNSTLKSL HQQSTQLSSS LTSVKTSLRS SLNDPLCLVH PSSETCNSIR LSLSQLNSNP ELRQLPPVDA ELDNVNNVLR TDLDGLVQQG YQSLNDIPDR VQRQTTTVVA GIKRVLNSIG SDIDNVTQRL PIQDILSAFS VYVNNTESYI HRNLPTLEEY D
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker
168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Form
Supplied as lyophilized powder from 20mM Tris, 500mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.01% sodium azide. Reconstitute with sterile ddH2O.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.