Recombinant protein corresponding to aa22~244 with an N-Terminal His-tag and GST-tag from Human SIAE expressed in E. coli. Swiss/UniProt Accession:Q9HAT2.
Amino Acid Sequence
AGIGFRFAS YINNDMVLQK EPAGAVIWGF GTPGATVTVT LRQGQETIMK KVTSVKAHSD TWMVVLDPMK PGGPFEVMAQ QTLEKINFTL RVHDVLFGDV WLCSGQSNMQ MTVLQIFNAT RELSNTAAYQ SVRILSVSPI QAEQELEDLV AVDLQWSKPT SENLGHGYFK YMSAVCWLFG RHLYDTLQYP IGLIASSWGG TPIEAWSSGR SLKACGVPKQ GSIP
Predicted Isoelectric Point
6.5
Predicted Molecular Weight
54.5kD
Accurate Molecular Weight
54kD as determined by SDS-PAGE reducing conditions
Subcellular Location
Secreted, Lyosome
Applications
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. Aliquots are stable for at least 12 months from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as lyophilized powder in PBS, pH7.4, 5% trehalose, 0.01% sarcosyl. Reconstitute in sterile 10mM PBS, pH7.4 to a concentration of 0.1-1.0mg-ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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