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171903 Hsp70 (Q435A), Recombinant, Human (Human induced Heat Shock Protein 70) CAS:

Specifications
References
Grade
Highly Purified
Swiss Prot
P08107
Accession Number
NM_005345
Molecular Weight
70.8
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

Human induced Heat Shock Protein 70 (Q435A), also known as induced Hsp70 and Hsp70i, with a Gln to Ala mutation on a.a.435 of the Hsp70 protein. GenBank Accession No. NM_005345, a.a.1-641(end) with C-terminal His-tag, MW=70.8kD, expressed in an E. coli expression system.

Source
Recombinant protein corresponding to aa1-641 of Hsp70 at the C-terminus, fused to His-tag expressed in E.coli.
Molecular Weight
~70.8kD
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectively profiling.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
E. coli
Purity
~90%
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween-20, 175mM Imidazole, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Lee K.J. et al., Exp. Mol. Med. 40 (6), 658-668 (2008). 2. Birtas-Atesoglu E.et al., Clin. Exp. Rheumatol. 26, S96-S98 (2008).
USBio References
No references available
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