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171908 IDH2, Recombinant, Human, aa1-452 (isocitrate dehydro-genase 2) CAS:

Specifications
References
Grade
Purified
Swiss Prot
P48735
Accession Number
NM_002168
Molecular Weight
52
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

Human isocitrate dehydro-genase 2, also known as IDH2, GenBank Accession No. NM_002168, a.a. 1-452(end) with with Arg to Gln mutation on a.a. 140 and C-terminal FLAG-tag, MW = 52kD, expressed in a baculovirus infected Sf9 cell expression system. Note: This protein runs aberrantly by SDS-PAGE

Source
Recombinant protein corresponding to aa1-452 from human IDH2 at C-terminal, fused to FLAG-tag expressed in baculovirus infected Sf9 cells.
Molecular Weight
~52kD
Specific Activity
61 pmol/min/ug
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
baculovirus infected Sf9 cells
Purity
~90%
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 80ug/ml FLAG peptide, 0.04% Tween-20, 20% glycerol
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Schaap, F.G., et al., Adv. Anat. Pathol. 2013;20(1):32-38. 2. Grassian, A.R., et al., J. Biol. Chem. 2012;287(50):42180-42194
USBio References
No references available
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