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218001 CD14, Recombinant, Human, aa20-349, His-Tag (Monocyte Differentiation Antigen CD14) CAS:

Specifications
References
Grade
Purified
Accession Number
NP_001167576
Molecular Weight
37.9
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.

Source
Recombinant protein corresponding to aa20-349 from human CD14, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight
~37.9kD (353aa), confirmed by MALDI-TOF
Applications
Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~90% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 30% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Kelley S.L., et al (2013). J. Immunol. 190:1304-1311. 2. Setoguchi M., et al (1989). Biochim. Biophys. Acta 1008:213-222.
USBio References
No references available
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