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218650 TSHB, aa21-138, Recombinant, Human, His-Tag (Thyrotropin Subunit beta Precursor) CAS:

Specifications
References
Grade
Purified
Accession Number
NP_000540
Molecular Weight
15.9
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. TSHB is the beta subunit of thyroid stimulating hormone. Mutations in this gene result in congenital hypothyroidism.

Source
Recombinant protein corresponding to aa21-138 from human TSHB, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight
~15.9kD (141aa)
Applications
Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~85% (SDS-PAGE)
Concentration
~0.1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Hayashizaki Y., Miyai K.. et al. (1985). FEBS Lett. 188:394-400.
USBio References
No references available
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