Suc-Leu-Leu-Val-Tyr-AMC CAS 94367-21-2

Subscribe to our newsletter!

Welcome | LOG OUT

226822 Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY-AMC, Proteasome Substrate, Calpain Substrate, Cathepsin B Substrate) CAS: 94367-21-2

Specifications

References

CAS Number

94367-21-2

Grade

Highly Purified

Molecular Formula

C40H53N5O10

Molecular Weight

763.9

EU Commodity Code

38220090

Shipping Temp

Blue Ice

Storage Temp

-20°C

Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome, calpains and other chymotrypsin-like proteases. This substrate is useful for inhibitor screening and kinetic analysis.

Excitation

Extinction: 380nm. Emission: 460nm.

Molecular Formula

C40H53N5O10

Molecular Weight

763.9

Sequence

N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin

Solubility

Soluble in DMSO.

Storage and Stability

Stable for at least 1 year after receipt when stored at -20°C.

Purity

≥95% (HPLC)

Form

Supplied as a solid lyophilized powder.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

1. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates: T. Sasaki, et al.; J. Biol. Chem. 259, 12489 (1984). 2. Kinetic characterization of the chymotryptic activity of the 20S proteasome: R.L. Stein, et al.; Biochemistry 35, 3899 (1996). 3. 26S proteasome activity is down-regulated in lung cancer stem-like cells propagated in vitro: J. Pan, et al.; PLoS One 5, e13298 (2010). 4. Low cell density induced epithelial-mesenchymal transition in MCF10A cell is regulated by proteasome activity: J. Fu, et al.; J. Oncobiomark. 1, 4 (2013). 5. Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013).