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349966 GTF2F2, Recombinant, Human, aa1-249, His-Tag (General transcription factor IIF subunit 2) CAS:

Specifications
References
Grade
Purified
Accession Number
NP_004119
Molecular Weight
30.5
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

GTF2F2, also known as TFIIF, is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.

Source
Recombinant protein corresponding to aa1-249 of human GTF2F2, fused to His-tag at N-terminal expressed in E. coli.
Molecular Weight
~30.5kD
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS SDKLSLEGIV VQRAECRPAA SENYMRLKRL QIEESSKPVR LSQQLDKVVT TNYKPVANHQ YNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK DLVDITKQPV VYLKEILKEI GVQNVKGIHK NTWELKPEYR HYQGEEKSD
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 1mM DTT, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Purrello M. et al. .(1995) Cytogenet Cell Genet. 69(1-2):75-80.
USBio References
No references available
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