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351080 ANAPC13, Recombinant, Human, aa1-74, T7-tag (Anaphase-promoting complex Subunit 13, APC13, SWM1) CAS:

Specifications
References
Grade
Purified
Accession Number
NP_056206
Molecular Weight
10
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C

ANAPC13, also known as anaphase-promoting complex subunit 13, is component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. This protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex.

Source
Recombinant protein corresponding to aa1-74 from human ANAPC13, fused to T7-tag at N-terminal expressed in E. coli.
Molecular Weight
~10kD (89aa)
AA Sequence
MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~90% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Schwickart M., et al. (2004) Mol. Cell. Biol. 24:3562-3576 2. Jin L., et al. (2008) Cell. 133:653-665
USBio References
No references available
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