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351205 EIF1AY, Recombinant, Human, aa1-144, His-Tag (Eukaryotic translation initiation factor 1A, Y-linked, eIF-1A Y Isoform, Eukaryotic translation initiation factor 4C, eIF-4C) CAS:

Specifications
References
Grade
Highly Purified
Accession Number
NP_004672
Molecular Weight
18.8
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

EIF1AY is similar to eukaryotic translation initiation factor 1A (EIF1A). This protein seems to be required for maximal rate of protein biosynthesis. It enhances ribosome dissociation into subunits and is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.

Recombinant protein corresponding to aa1-144 from human EIF1AY, fused to His-tag at N-terminal expressed in E. coli.
Molecular Weight
~18.8kD (167aa)
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPKNKGK GGKNRRRGKN ENESEKRELV FKEDGQEYAQ VIKMLGNGRL EALCFDGVKR LCHIRGKLRK KVWINTSDII LVGLRDYQDN KADVILKYNA DEARSLKAYG ELPEHAKINE TDTFGPGDDD EIQFDDIGDD DEDIDDI
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥95% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 100 mM sodium chloride, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Mahbybyl Huq AH., et al. (2009) J Child Neurol. 24(10):1258-61. 2. Page DC., et al (1997) Science. 278(5338):675-80.
USBio References
No references available
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