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351421 PECAM1, Recombinant, Human, aa28-601, His-Tag (Platelet endothelial cell adhesion molecule, CD31, CD31/EndoCAM, endoCAM, GPIIA, PECA1, PECAM-1) CAS:

Specifications
References
Grade
Highly Purified
Swiss Prot
P16284
Accession Number
NP_000433
Molecular Weight
65.5
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

PECAM1, also known as platelet endothelial cell adhesion molecule, induces susceptibility to atherosclerosis. It prevents phagocyte ingestion of closely apposed viable cells by transmitting ‘detachment’ signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes. The encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte.

Recombinant protein corresponding to aa28-601 from human PECAM1, fused to His-tag at C-terminal expressed in insect cell.
Molecular Weight
~65.5kD (582aa)
Amino Acid Sequence
QENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQVLV EGVPSPRVTL DKKEAIQGGI VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQVTHLAQEF PEIIIQKDKA IVAHNRHGNK AVYSVMAMVE HSGNYTCKVE SSRISKVSSI VVNITELFSK PELESSFTHL DQGERLNLSC SIPGAPPANF TIQKEDTIVS QTQDFTKIAS KSDSGTYICT AGIDKVVKKS NTVQIVVCEM LSQPRISYDA QFEVIKGQTI EVRCESISGT LPISYQLLKT SKVLENSTKN SNDPAVFKDN PTEDVEYQCV ADNCHSHAKM LSEVLRVKVI APVDEVQISI LSSKVVESGE DIVLQCAVNE GSGPITYKFY REKEGKPFYQ MTSNATQAFW TKQKASKEQE GEYYCTAFNR ANHASSVPRS KILTVRVILA PWKKVEHHHH HH
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, Baculovirus
Concentration
~0.1mg/ml
Form
Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Brown S., et al. (2002) Nature. 418:200-203. 2. Dasgupta B., et al. (2009) J Immunol. 182: 5041-5051.
USBio References
No references available
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