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374413 NFYB, Recombinant, Human, aa1-207, GST-Tag (Nuclear Transcription Factor Y Subunit beta) CAS:

Specifications
References
Grade
Purified
Swiss Prot
P25208
Molecular Weight
49.8
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Source
Recombinant protein corresponding to aa1-207 from human NFYB, fused to GST-Tag at N-terminal, expressed in E. coli.
Molecular Weight
~49.8kD
Amino Acid Sequence
MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Concentration
As Reported
Form
Supplied as a liquid in Tris, 50% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. "Intron-exon organization of the NF-Y genes. Tissue-specific splicing modifies an activation domain." Li X.-Y., Hooft van Huijsduijnen R., Mantovani R., Benoist C.O., Mathis D. J. Biol. Chem. 267:8984-8990(1992).
USBio References
No references available
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