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375023 REG3A, Recombinant, Human, aa27-175, GST-Tag (Regenerating Islet-derived Protein 3-alpha) CAS:

Specifications
References
Grade
Purified
Swiss Prot
Q06141
Molecular Weight
43.6
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
REG3A, HIP, PAP, PAP1, Regenerating islet-derived protein 3-alpha, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.

Source
Recombinant protein corresponding to aa27-175 from human REG3A, fused to GST-Tag at N-terminal, expressed in E. coli.
Molecular Weight
~43.6kD
Amino Acid Sequence
EEPQRELPSARIRCPKGSKAYGSHCYALFLSPKSWTDADLACQKRPSGNLVSVLSGAEGSFVSSLVKSIGNSYSYVWIGLHDPTQGTEPNGEGWEWSSSDVMNYFAWERNPSTISSPGHCASLSRSTAFLRWKDYNCNVRLPYVCKFTD
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Form
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Molecular basis for peptidoglycan recognition by a bactericidal lectin.Lehotzky R.E., Partch C.L., Mukherjee S., Cash H.L., Goldman W.E., Gardner K.H., Hooper L.V.Proc. Natl. Acad. Sci. U.S.A. 107:7722-7727(2010).
USBio References
No references available
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