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378001 UMPS, Recombinant, Human, aa1-480, His-Tag (Uridine 5'-monophosphate Synthase, OPRT) CAS:

Specifications
References
Grade
Highly Purified
Accession Number
NP_000364
Molecular Weight
53
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

UMPS, also known as uridine 5'-monophosphate synthase, is a bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis. It is an indispensable component in this metabolic pathway and is a target for antimalarial and antitumor drugs. Its expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but It in the normal tissues can help predict the toxicities associated with 5-FU.

Source
Recombinant protein corresponding to aa1-480 from human UMPS, fused to His-Tag at C-terminal, expressed in insect cell.
Molecular Weight
~53kD (486aa) 50-70kD (SDS-PAGE under reducing conditions)
Endotoxin
<1EU/1ug (determined by LAL method)
AA Sequence
MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV HHHHHH
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, Baculovirus
Purity
~95% (SDS-PAGE)
Concentration
~0.1mg/ml
Form
Supplied as a liquid in PBS, pH 7.4
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Zhang Y., et al. (2013) J Biol Chem. 288:34746-34754.|2. Dong Q., et al. (2012) Nan Fang Yi Ke Da Xue Xue Bao. 32:1179-1181.
USBio References
No references available
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