Collagenase is prepared to contain lower proteolytic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suitable for use in mammary primary cell isolation.

Type 1: Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells. Type 2: Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells. Type 3: Containing low proteolytic activity. It is usually used for mammary and fetal cells. Type 4: Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.

≥100 units/mg dry weight.

≥50

≤3

≤0.3

As reported

1 unit releases 1 µmole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.

SDS-PAGE

Suitable for use in mammary primary cell isolation. Collagenase is prepared to contain lower proteolytic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity.

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clostridium histolyticum

Supplied as a dialyzed, lyophilized powder.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.