GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y.
GSTA1: The alpha gene cluster consists of 5 genes GSTA1-GSTA5; the GSTA1 contains 222aa (chr 6p12), widely expressed in human tissues of liver, kidney, testis, adrenal and pancrease. GST alpha 1- 1 concentration as a marker for hepatocellular impairment, GSTA1 concentration in blood is a more specific marker than AST or ALT, and has a shorter half-life than ALT The GSTA1 shows enzyme activity towards CDNR; NBD-CI; delta 5 AD, PGE2 AND PGF2a synthase.
Source
Human GST-alpha/GST A1-1 fusion protein with N-terminal His-tag (25kD), expressed in E. coli.
Molecular Weight
Under native conditions, GST-alpha is homodimeric protein of ~50kD and under reducing conditions ~25kD.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 10ul/lane. Heat once prior to loading on gels. The loading volume may be adjusted if necessary. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Highly Purified (~95%)
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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