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G8140-14D Glyceraldehyde 3-Phosphate Dehydrogenase, Recombinant, Human (GAPD, GAPDH, G3PD, MGC88685) CAS:

Specifications
References
Grade
Purified
Specificity
=
Applications
WB
Accession Number
NP_002037
Molecular Weight
36335
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kD subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair.

Recombinant GAPDH protein, 1-335aa, was expressed in E. coli and purified by using conventional chromatography techniques.
Molecular Weight: 36kD (335 aa)
Sequence: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
Enzyme Activity
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Source
Recombinant, E. coli
Purity
≥ 95% by SDS-PAGE
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris pH 8.0, 1mM EDTA, 1mM DTT, 20% glycerol.
Specificity
=
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Tso JY et al (1985) Nucl. Acid Res. 13, 2485-2502;|2.Hanuer A et al (1984) EMBO J. 3, 2627-2633;|3. Arcari P et al (1984) Nucl. Acid. Res. 12, 9179-9189;|4. Meyer-Sigler K et al (1991) PNAS 88, 8460-8464;|5. Tokunaga K et al (1987) Cancer Res. 47, 5616-5619;|6. Allen RW et al (1987) JBC 262, 649-653|7. Ralser M., et al. (2007).J Biol 21;6(4):10|8. Tisdale Ej., et al. (2007).Traffic. 8;(6):733-41
USBio References
No references available
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