DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells.

Theoretical Peptide Sequence: YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

ED50 of beta NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.

Suitable for use in Western Blot. Other applications not tested.

Western Blot: The NGF protein migrates at ~12-16kD in SDS-PAGE. It has a predicted molecular mass of 13.5kD. The protein separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. The unmodified protein has a predicted pI of 9. Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant, Modified human 293 cells

~95% (SDS-PAGE, silver stain)

Not Determined

Supplied as lyophilized powder from PBS, 1% HSA, 10% trehalose. Reconstitute with sterile PBS.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.