Nudix-type Motif 21, aa1-227, Recombinant, Human CAS

Grade

Purified

Accession Number

NP_008937

Molecular Weight

28.3

EU Commodity Code

30021019

Shipping Temp

Blue Ice

Storage Temp

-20°C

Nudix (Nucleoside Diphosphate Linked Moiety X)-type Motif 21, DKFZp686H1588

NUDT21, also known as CPSF5 or CFIm25, is a member of the Nudix hydrolase family of pyrophosphatases. This protein localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NUDT21 is the smaller subunit of the complex and is present in all heterodimer combinations. NUDT21 plays an important role in pre-mRNA 3' cleavage and polyadenylation processing.

Source

Recombinant corresponding to human NUDT21, fused to His-tag at N-terminus, expressed in E. coli.

Molecular Weight

~28.3kD

Sequences of Amino Acids

MGSSHHHHHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

Recombinant, E. coli

Purity

≥90% (SDS-PAGE)

Concentration

~1mg/ml

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 20% glycerol.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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Nudix (Nucleoside Diphosphate Linked Moiety X)-type Motif 21, DKFZp686H1588

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