Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation of A to T in the Clock gene affects circadian rhythmicity in mice. Clock has been mapped to chromosome 5. Mouse Clock encodes a transcription factor, a single polypeptide chain of 855aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846aa). Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney). Although, Clock is constitutively expressed (not rhythmic) in the SCN. It may still be an important component of circadian machinery.

Suitable for use in ELISA and Antibody Blocking. Not suitable for ues in Western Blot due to low molecular weight. Other applications not tested.

ELISA: 50-100ng control peptide/well Antibody Blocking: 5-10ug per 1ul P3342-01B (antiserum) or per 1ug P3342-01 (affinity purified antibody). Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Drosophilla synthetic peptide

Highly purified.

~1mg/ml

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.