SHP/SHPTP are tyrosine phosphatases which contain SH2 domains. SHP 1/SHPTP is expressed primarily in hematopoietic cells, and binds specific phosphotyrosine residues, often in ITIM motifs. SHP 1 is a down-regulator of receptor signaling and inhibits activation of macrophages, B-cells, T-cells, and mast cells. Motheaten mice harbor a mutation in SHP 1, which leads to progressive inflammation. SHP 2 has a broader expression pattern, and appears to promote cell proliferation and differentiation. Additionally, SHP 2 regulates cell spreading and migration in a manner similar to FAK, suggesting that the two act together to dynamically remodel focal adhesions.
Recombinant protein corresponding to aa224-529 in the catalytic domain of human SHP 2 with N-Terminal GST-Tag, expressed in an E. coli expression system. GenBank Accession: NM_002834
Specific Activity
~3U/ug.
Unit Definition
One unit will hydrolyze 1nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C
Assay Buffer
50mM HEPES, pH 7.4, 2mM EDTA, 3mM DTT, 100mM sodium chloride, 50mM pNPP. Incubate for 20 minutes at 30ºC. Absorbance is measured at 405nm.
Applications
Sutiable for use in Enzyme Kinetics, Screening inhibitors and Selectivity Profiling. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Also Available
S1013-29: SHP 2 (SHPTP-2) Pab Rb xMo S1013-29A: SHP 2 Pab Rb xHu S1013-29B: SHP 2 (224-529), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (SHPTP-2, PTPN11) S1013-29E: SHP 2, phosphorylated (Tyr542) Pab Rb xHu S1013-29F: SHP 2, phosphorylated (Tyr580) Pab Rb xHu
Source
Human, recombinant protein expressed E. coli
Form
Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 150mM sodium chloride, 0.05% Tween 20, 2mM EDTA, 1mM DTT, 10mM glutathione, 50% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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