Technical Data
4-1 BB Ligand, Recombinant, Human (4-1BBL, CD137L, TNFSF9, Tumor necrosis factor ligand superfamily member 9)
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Human 4-1BB Ligand (4-1BBL, CD137L) is a 32kD type II transmembrane protein that belongs to TNF superfamily (TNFSF) of molecules.1-4 The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25aa N-terminal cytoplasmic domain, a 23aa transmembrane segment, and a 206aa C-terminal extracellular region.5 The extracellular domain (ECD) of 4-1BBL has a jelly-roll, -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated Tcells, neurons and astrocytes.2, 3, 6 A 26kD soluble form of 4-1BBL is known to occur in human. Although it is presumably generated by MMP activity, its amino acid size is currently unreported.4 The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF- ) by monocytes.7, 8 4-1BBL binding to CD137/4-1BB produces a number of effects. Its seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN- in CD4+ cells, and IFN- in CD8+ cells.9, 10

Source: Recombinant, Human from E. coli

Endotoxin: 0.1ng/ug (1EU/ug)

Biological Activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC (peripheral blood mononuclear cells). The ED50 for this effect is 5-10ng/ml.

Please Note: Results may vary with PBMC (peripheral blood mononuclear cells) donors.

Reconstitution: We recommend a quick spin followed by reconstitution in water to
a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

Stability: The Lyophilized protein is stable for a few weeks at room temperature, but best stored at -20C. Reconstituted 4-1BBL should be storred in working aliquots at -20C.

Country of Origin: USA

Molecular Weight:
Source: Recombinant, Human from E. coli
Purity: 98% by SDS-PAGE and HPLC.
Form: The sterile filtered solution was lyophilized from 5mM Tris, pH 8.0, 75mM sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kwon, B. et al., 2003, Exp. Mol. Med. 35:8.
2. Salih, H.R. et al., 2002, Int. J. Clin. Pharmacol. Ther. 40:348.
3. Vinay, D.S. and B.S. Kwon, 1998, Semin. Immunol. 1:481.
4. Salih, H.R. et al., 2001, J. Immunol. 167:4059.
5. Alderson, M.R. et al., 1994, Eur. J. Immunol. 24:2219.
6. Reali, C. et al., 2003, J. Neurosci. Res. 74:67.
7. Michel, J. et al., 1999, Immunology 98:42.
8. Langstein, J. et al., 1998, J. Immunol. 160:2488.
9. Wen, T. et al., 2002, J. Immunol. 168:4897.
10. Dawicki, W. and T.H. Watts, 2004, Eur. J. Immunol. 34:743.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.