Technical Data
0004-01E
4-1BB Ligand (4-1BBL) (APC)
Description:
4-1BB Ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that other TNFSF members, but shares only low aa sequence homology (14-16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart.1, 2 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines.3 A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases.4 By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as non-covalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecules that enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.3

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Use 10ul to label 10e6 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b8H117Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceMouseRat
Concentration:
As reported
Immunogen:
Sf21-derived rm4-1BB Ligand extracellular domain
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with APC.
Specificity:
Recognizes mouse 4-1BB Ligand/TNFSF9.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goodwin, R.G. et al., 1993, Eur. J. Immunol. 23:2631. 2. Alderson, M.R. et al., 1994, Eur. J. Immunol. 24:2219. 3. Kwon, B., H.W. Lee and B.S Kwon, 2002, TRENDS in Immunology 23:378. 4. Salih, H.R. et al., 2001, J. Immunol. 167:4059.