Technical Data
0004-01F
4-1BB Ligand (4-1BBL) (Carboxyfluorescein)
Description:
4-1BB Ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that other TNFSF members, but shares only low aa sequence homology (14-16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart.1, 2 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines.3 A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases.4 By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as non-covalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecules that enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.3

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: use 10ul to label 10e6 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8H117Affinity Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceMouseRat
Concentration:
As reported
Immunogen:
Sf21-derived rm4-1BB Ligand extracellular domain
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Carboxyfluorescein.
Specificity:
Recognizes mouse 4-1BB Ligand.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goodwin, R.G. et al., 1993, Eur. J. Immunol. 23:2631. 2. Alderson, M.R. et al., 1994, Eur. J. Immunol. 24:2219. 3. Kwon, B., H.W. Lee and B.S Kwon, 2002, TRENDS in Immunology 23:378. 4. Salih, H.R. et al., 2001, J. Immunol. 167:4059.