Technical Data
0004-01H
4-1BB LIGAND (TNFSF9, 4-1BBL, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9)
Description:
TNFSF9, a type II transmembrane glycoprotein and member of the TNF superfamily, preferentially expressed by B cells, which acts as a ligand for CD137 (4-1BB ligand receptor/TNFRSF9). Interaction between 4-1BBL and its receptor acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells (Tc) and in the process of antigen presentation. Expression of 4-1BBL by a variety of carcinoma cells, suggests a role for 4-1BBL in tumor cell-T cell interactions.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-2ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
Goat Anti Rabbit IgG (H&L) HRP
Sheep Anti Rabbit IgG Biotin
Goat Anti Rabbit IgG (Fc) FITC, HRP
Goat Anti Rabbit IgG (H&L) HRP
Sheep Anti Rabbit IgG FITC
Sheep Anti Rabbit IgG RPE
Sheep Anti Rabbit IgG DyLight™488, DyLight™549, DyLight™649
Sheep Anti Rabbit IgG HRP

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 100ul dH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For long term storage the addition of 0.09% sodium azide is recommended. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant human 4-1BBL.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in 100ul distilled water.
Specificity:
Recognizes human 4-1BB Ligand, otherwise known as TNFSF9
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.