Technical Data
0004-01I
4-1BB LIGAND (TNFSF9, 4-1BBL, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9) (Biotin)
Description:
TNFSF9, a type II transmembrane glycoprotein and member of the TNF superfamily, preferentially expressed by B cells, acts as a ligand for CD137 (4-1BB ligand receptor/TNFRSF9).
Interaction between 4-1BBL and its receptor acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells (Tc) and in the process of antigen presentation. Expression of 4-1BBL by a variety of carcinoma cells, suggests a role for 4-1BBL in tumor cell-T cell interactions.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.25-1ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
Streptavidin APC
Streptavidin FITC
Streptavidin TRITC
Streptavidin RPE, RPE
Streptavidin HRP
Streptavidin Alk. Phos.

Storage and Stability:
Lyophilized powder may be stored at-20C. Stable for 12 months at -20C. Reconstitute with 0.5ml sterile PBS containing 0.1% BSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Recombinant human 4-1BBL.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 0.5ml sterile PBS containing 0.1% BSA.
Specificity:
Recognizes human 4-1BB Ligand (TNFSF9, 4-1BBL, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.